《植物生理学报》 2010, 46(8): 779-782

巴戟天离体苗不定根发育的观察

林美珍¹, 郑松², 邓炜², 田惠桥^2,*

1 漳州卫生职业学院药学系, 福建漳州 363000; 2 厦门大学生命科学学院, 福建厦门 361005

通信作者：田惠桥；E-mail: hqtian@xmu.edu.cn；Tel: 0592-2186486

摘　要：

以巴戟天幼嫩种子中的胚为外植体进行组织培养诱导出愈伤组织, 继而诱导分化出芽, 长成小苗。将小苗转入生根培养基中, 从茎基部诱导出不定根, 再生植株。对离体苗的不定根发育进行结构观察, 发现巴戟天组培苗的不定根是由形成层细胞先分裂分化形成根原基后发育而成的。

关键词：巴戟天; 组织培养; 不定根

收稿：2010-02-02   修定：2010-04-13

资助：福建省卫生厅医学创新课题(09-CXB-43)和福建省漳州市科技局课题(Z2008038)。

Observation on the Development of in vitro Adventitious Roots of Morinda officinalis How.

LIN Mei-Zhen¹, ZHENG Song², DENG Wei², TIAN Hui-Qiao^2,*

¹Department of Pharmacy, Zhangzhou Health Vocational College, Zhangzhou, Fujian 363000, China; ²School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China

Corresponding author: TIAN Hui-Qiao; E-mail: hqtian@xmu.edu.cn; Tel: 0592-2186486

Abstract:

Callus was induced from immature embryo of Morinda officinalis and then buds were induced from the callus. When the buds grew up, the stems were transplanted in a rooting medium to induce adventitious roots. The generation and the growth of adventitious roots were observed with paraffin method. The in vitro adventitious roots came from the cambial cells in culture.

Key words: Morinda officinalis; tissue culture; adventitious root